Composition and diversity of arbuscular mycorrhizal fungi spore associated with different land-use types in tropical gold mine


  • Faisal Danu Tuheteru Jurusan Kehutanan, Universitas Halu Oleo
  • H Husna Department of Forestry, Faculty of Forestry and Environmental Science, Halu Oleo University, Kendari, Southeast Sulawesi 93121, Indonesia
  • A Albasri
  • Asrianti Arif
  • Kartini Kramadibrata
  • Geofrey Soka Department of Wildlife Management, Sokoine University of Agriculture, P.O. Box 3073, Morogoro, Tanzania



arbuscular mycorrhizal fungi, Glomeraceae, gold tailings, land-use, Indonesia


Understanding the composition and diversity of arbuscular mycorrhizal fungi (AMF) is imperative for potentially enhancing their ecological role in different terrestrial ecosystems. Land use can have substantial effects on AMF species composition and diversity, but such effects have been explored less in tropical landscapes. In this study, we assessed the effects of disturbances on AMF species richness, observed the potential development of AMF types to produce mycorrhizal biofertilizer bioinoculants.  This study was conducted identifying and selecting AMFs was for the purpose of managing post-mining land in Bombana District, Southeast Sulawesi, Indonesia.  AMF spores collected from the field and trap culture were directly isolated and morphologically identified. A total of 15 AMF species were identified, including 11 species from field samples and 9 species from trap cultures. We noted that five AMF species were unique to field conditions and 5 AMF species were uniquely isolated from trap culture.  It appears that Glomeraceae family contributed the highest number of species in all land-use types.  Glomus sp. 1 was the most frequent species found in all land-use types. The Simpson’s index, Shannon index and evenness ranged from 1.60 ± 0.51 to 2.40 ± 0.40; 0.41 ± 0.17 to 0.62 ± 0.17; 0.25 ± 0.10 to 0.39 ± 0.11, respectively. In this study, we found three new records of AMF species including Entrophospora colombiana, Sclerocystis microcarpa and Glomus coronatum for Indonesia, i.e. from this study, it is clear that different land-use types affected AMF spore composition and species diversity.  All AMF species found in this study were then applied to the land to improve land quality.


Bainard, L.D., Dai, M., Furrazola-Gómez, E., Torres-Arias, Y., Bainard, J.D., Sheng, M., Eilers ,W. and Hamel, C. 2015. Arbuscular mycorrhizal fungal communities are influenced by agricultural land use and not soil type among the chernozem great groups of the Canadian Prairies. Plant and Soil 387: 351–362

Bainard, L.D., Koch, A.M., Gordon, A.M. and Klironomos, J.N. 2012. Temporal and compositional diferences of arbuscular mycorrhizal fungal communities in conventional monocropping and tree-based intercropping systems. Soil Biology and Biochemistry 45: 172–180.

Brundrett, M., Bougher, N., Dell, B., Grove, T. and Majalaczuk. 1996. Working with Mycorrhizas In Forestry and Agriculture. Australian Centre for International Agriculture Research, Canberra.

Buck, M.T., Straker, C.J., Mavri-Damelin, D. and Weiersby, I.M. 2019. Diversity of arbuscular mycorrhizal (AM) fungi colonising roots of indigenous Vachellia and Senegalia trees on gold and uranium mine tailings in South Africa. South African Journal of Botany 121: 34–44

Chiomento, J.L.T., Stürmer, S.L., Carrenho, R., da Costa, R.C., Scheffer-Basso, S.M., Antunes, L.E.C., Nienow, A.A. and Calvete, E.O. 2019. Composition of arbuscular mycorrhizal fungi communities signals generalist species in soils cultivated with strawberry. Scientia Horticulturae 253: 286–294

Dandan, Z. and Zhiwei, Z. 2007. Diversity of arbuscular mycorrhizal fungi in the hot-dry valley of the Jinsha River, Southwest China. Applied Soil Ecology 37(1):118-128.

Gai, J.P., Tian, H., Yang, F.Y., Christie, P., Li, X.L. and Klironomos, J.N. 2012. Arbuscular mycorrhizal fungal diversity along a Tibetan elevation gradient. Pedobiologia 55:145– 151

Govindan, M., Rajeshkumar, P.P., Varma, C.K.Y., Anees, C.K.M., Rashmi, C.R. and Nair, A.B. 2019. Arbuscular Mycorrhizal Fungi Status of Mango (Mangifera indica) Cultivars Grown in Typic Quartzipsamments Soil. Agricultural Research 9: 188-196

Guadarrama, P. and Ãlvarez-Sánchez, F.J. 1999. Abundance of arbuscular mycorrhizal fungi spores in different environments in a tropical rain forest, Veracruz, Mexico. Mycorrhiza 8(5): 267-270

He, W., Fan, X., Zhou, Z., Zhang, H., Gao, X., Song, F. and Geng, G. 2019. The effect of Rhizophagus irregularis on salt stress tolerance of Elaeagnus angustifolia roots. Journal of Forestry Research Published online 11 Oktober 2019.

Husna, Budi, S.W.R, Mansur, I. and Kusmana, C. 2015. Diversity of arbuscular mycorrhizal fungi in the growth habitat of kayu kuku (Pericopsis mooniana Thw.) in Southeast Sulawesi. Pakistan Journal of Biological Sciences 18(1): 1-10.

Husna, Tuheteru, F.D. and Arif, A. 2018. Arbuscular Mycorrhizal Fungi Symbiosis and Conservation of Endangered Tropical Legume Trees. In : B. Giri et al. (eds.), Root Biology, Soil Biology 52. Springer. Germany.

Husna, Mansur, I., Budi, S.W.R, Tuheteru, F.D., Arif, A., Tuheteru, E.J. and Albasri. 2019. Effects of arbuscular mycorrhizal fungi and organic material on growth and nutrient uptake by Pericopsis mooniana in coal mine. Asian Journal of Plant Science 18(3): 101-109

Johnson, J.M, Houngnandan P., Kane A., Sanon, K.B. and Neyra M. 2013. Diversity patterns of indigenous arbuscular mycorrhizal fungi associated with rhizosphere of cowpea (Vigna unguiculata (L.) Walp.) in Benin, West Africa. Pedobiologia 56:121-128

Kivlin, S.N., Christine, V.H. and Treseder, K.K. 2011. Global diversity and distribution of arbuscular mycorrhizal fungi. Soil Biol Biochem 43:2294-2303

Koske, R.E., Gemma, J.N. and Jackson, N. 1997. Arbuscular mycorrhizal fungi associated with three species of turfgrass. Can J Bot 75:320–332

Kramadibrata K. 2012. Arbuscular fungi in Ujung Kulon National Park. Berita Biologi, 11 (2): 205-209 (in Indonesian).

Lekberg Y., Koide R.T., Rohr, J.R., Aldrich-Wolfe L. and Morton J.B. 2007. Role of niche restrictions and dispersal in the composition of arbuscular mycorrhizal fungal communities. J Ecol 95:95–105

Lovelock, C., Andersen, K.M. and Morton, J.B. 2003. Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment. Oecologia 135(2):268-79

Meharg, A.A. and Cairney, J.W.G. 1999. Co-evolution of mycorrhizal symbionts and their hosts to metal-contaminated environments. Advances in Ecological Research 30, 69-112.

Muchane, M.M., Mugoya, C and Masiga C.W. 2012. Effect of land use system on Arbuscular Mycorrhiza fungi in Maasai Mara ecosystem, Kenya. Afr. J. Microbiol. Res. 6, 3904-3916.

Oehl, F., Laczko, E., Bogenrieder, A., Stahr, K., Bösch R., Van der Heijden M. and Sieverding, E. 2010. Soil type and land use intensity determine the composition of arbuscular mycorrhizal fungal communities. Soil Biol. Biochem. 42, 724–738.

Oehl F, Sieverding E., Ineichen K., Ris E-A, Boller T. and Wiemken A. 2005. Community structure of arbuscular mycorrhizal fungi at different soil depths in extensively and intensively managed agroecosystems. New Phytol 165:273–283

Pacioni G. 1992. Wet sieving and decanting techniques for the extraction of spores of VA mycorrhizal fungi. in : Norris, J.R., D.J. Read and A.K Varma (Eds.). Methods in Microbiology. Vol. 24. Academic Press Inc. Sandiego.

Phillips, J.M. and Hayman, D.S. 1970. Improved procedures for clearing and staining parasitic and vesicular-arbuscular mycorrhizae fungi for rapid assessment of infection. Trans Br Mycol Soc 55: 158-161.

Prasetyo, B., Krisnayanti, B.D., Utomo, W.H. and Anderson, C.W.N. 2010. Rehabilitation of Artisanal Mining Gold Land in West Lombok, Indonesia: 2. Arbuscular Mycorrhiza Status of Tailings and Surrounding Soils. J Agric Sci 2(2) : 202-209.

Redecker, D., Schüßler, A., Stockinger, H., Stürmer, S.L., Morton, J.B. and Walker C. 2013. An evidence-based consensus for the classification of arbuscular mycorrhizal fungi (Glomeromycota). Mycorrhiza. 23:515-531.

Schüßler, A. and Walker C. 2010. The Glomeromycota. A species list with new families and new genera. Kew: The Royal Botanic Garden Kew

Shi, Z.Y., Chen, Y.L., Feng, G., Liu, R.J., Christie, P and Li, X.L. 2006. Arbuscular mycorrhizal fungi associated with the Meliaceae on In Haiman Island, Cina. Mycorrhiza 16(2):81-87

Shukla, A., Vyas, D. and Anuradha, J. 2013. Soil depth: an overriding factor for distribution of arbuscular mycorrhizal fungi. J Soil Sci Plant Nutr 13(1):23-33

Singh, A.K. and Jamaluddin. 2011. Status and diversity of arbuscular mycorrhizal fungi and its role innatural regeneration on limestone mined spoils. Biodiversitas 12 (2) :107-111

Smith, S.E. and Read, D.J. 2008. Mycorrhizal symbiosis. Third ed. Academic Press. San Diego.

Soka, G.E. and Ritchie, M.E. 2018. Arbuscular mycorrhizal spore composition and diversity associated with different land uses in a tropical savanna landscape, Tanzania. Appl Soil Ecol 125: 222-232

Stürmer, S.L., Kemmelmeier, K., Moreira, B.C., Catarina, M., Gilmara, M.K., Pereira, M.D and da Silva, K. 2018. Arbuscular mycorrhizal fungi (Glomeromycota) communities in tropical savannas of Roraima, Brazil. Mycol Prog 17(10): 1149-1159

Suharno, Soetarto, E.S., Sancayaningsih, R.P. and Kasiamdari, R.S. 2017. Association of arbuscular mycorrhizal fungi (AMF) with Brachiaria precumbens (Poaceae) in tailing and its potential to increase the growth of maize (Zea mays). Biodiversitas 18: 433-441

Suharno, Kasiamdari, R.S., Soetarto, E.S and Sancayaningsih, R.P. 2016. Presence of arbuscular mycorrhizal fungi on fern from tailing deposition area of gold mine in Timika, Indonesia. International Journal of Environmental Bioremediation & Biodegradation, 4 (1), 1-7.

Tchabi A, Coyne, D., Hountondji, F., Lawouin, L., Wiemken, A. and Oehl, F. 2008. Arbuscular mycorrhizal fungal communities in sub-Saharan Savannas of Benin, West Africa, as affected by agricultural land use intensity and ecological zone. Mycorrhiza 18:181–195

Tuheteru, F.D., Kusmana, C., Mansur, I., Iskandar. and, Tuheteru E.J. 2016. Potential of lonkida (Nauclea orientalis L.) for phytoremediation of acid mined drainage at PT. Bukit Asam Tbk. (Persero), Indonesia. Res J Bot 11:9-17

Tuheteru, F.D. and Wu, Q.S. 2017. Arbuscular mycorrhizal fungi and tolerance of waterlogging stress in plants. In : QS Wu (eds.) Arbuscular Mycorrhizas and Stress Tolerance of Plants. Springer. Singapore.

Tuheteru, F.D., Arif, A., Husna, Mansur, I., Tuheteru E.J, Jusniar, Basrudin, Albasri, Hadijah M.H, and Karepesina S. 2020. Arbuscular mycorrhizal fungal inoculation improves Nauclea orientalis L. growth dan phosphorus uptake in gold mine tailings soil media. J. Degrade. Min. Land Manage., 7 (3): 2193-2200

Uhlmann, E., Görke, C., Petersen, A. and Berwinkler, F.O. 2004. Comparison of species diversity of arbuscular mycorrhizal fungi in winter-rainfall areas of South Africa and summer-rainfall areas of Namibia. Mycological Progress. 3 (4): 267–274

Wang, F. 2017. Occurrence of arbuscular mycorrhizal fungi in mining-impacted sites and their contribution to ecological restoration : Mechanisms and applications. Crtical Reviews in Enviromental Science and Technology, 0 (0): 1-57

Xiang, D., Verbruggen, E., Hu, Y., Veresoglou, S.D., Rillig, M.C., Zhou, W., Xu, T., Li, H., Hao, Z., Chen, Y. and Chen, B. 2014. Land use influences arbuscular mycorrhizal fungal communities in the farming–pastoral ecotone of northern China. New Phytologist 204: 968-978

Zhang, Z., Zhang, J., Xu, Zhou, L. and Li, Y. 2019. Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Zenia insignis seedlings under drought stress. New Forest 50(4): 593-604

Zu Y, Ping, Y., Mu, L. and Yang, T. 2018. The diversity of arbuscular mycorrhizal fungi of Rosa acicularis ‘Luhe’ in saline areas. Journal of Forestry Research. 30(4): 1507-1512








How to Cite

Tuheteru, F. D., Husna, H., Albasri, A., Arif, A., Kramadibrata, K., & Soka, G. (2020). Composition and diversity of arbuscular mycorrhizal fungi spore associated with different land-use types in tropical gold mine. Journal of Degraded and Mining Lands Management, 8(1), 2503–2512.



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