Diversity of arbuscular mycorrhizal fungi in the rhizosphere of Plantago coronopus in Northwestern Algerian coast


  • Souad Tabti Laboratory of plant Biodiversity: conservation and valorisation, life and nature sciences Faculty, Djilali Liabès University, Sidi Bel Abbès, 22000, Algeria.
  • Fatima Zohra Bendimered-Mouri Laboratory of plant Biodiversity: conservation and valorisation, life and nature sciences Faculty, Djilali Liabès University, Sidi Bel Abbès, 22000, Algeria.




arbuscular mycorrhizal fungi, Glomeraceae, littoral dune, Plantago coronopus L.


Mycorrhizal fungi play a major role in the functioning of ecosystems. However, their identification has remained a challenge for scientific research. This study presents the first identification report of species of arbuscular mycorrhizal fungi in the rhizosphere of the halophyte Plantago coronopus L. in Algeria. Samples of rhizospheric soil were collected in spring 2018 at three sites in the Bomo-plage dunes west of Oran, Algeria. The spores were isolated by wet sieving, morphologically identified, and quantified. The mean spore density was 107.94 spores 100 g-1 dry soil, which is high compared to other dune ecosystems. Endomycorrhizal spore morphotypes were involved in the following Genus: Glomus, Septoglomus, Rhizophagus, Diversispora, Funneliformis, Dentiscutata, Claroideoglomus, Scutellospora, and Entrophospora, to the following Family: Glomeraceae, Gigasporaceae, Diversisporaceae, Claroideoglomeraceae, and Acaulosporaceae. The Glomeraceae was the most dominant identified family. The identification of indigenous arbuscular mycorrhizal fungi has been shown to be essential for future programs to restore disturbed dune ecosystems.


Acosta, A., Ercole, S., Stanisci, A., Pillar, V.D.P. and Blasi, C. 2007. Coastal Vegetation Zonation and Dune Morphology in Some Mediterranean Ecosystems. Journal of Coastal Research 236:1518-1524, doi:10.2112/05-0589.1.

Bago, B., Pfeffer, P.E. and Shachar-Hill, Y. 2000. Carbon metabolism and transport in arbuscular mycorrhizas. Plant Physiology 124:949-958, doi:10.1104/pp.124.3.949.

Barea, J.M., Palenzuela, J., Cornejo, P., Sanchez-Castro, I., Navarro-Fernandez, C., Lopez-Garcia, A., Estrada, B., Azcon, R., Ferrol, N. and Azcon-Aguilar, C. 2011. Ecological and functional roles of mycorrhizas in semi-arid ecosystems of Southeast Spain. Journal of Arid Environments 75:1292-1301, doi:10.1016/j.jaridenv.2011.06.001.

Becerra, A.G., Cofré, M.N. and García I. 2019. Arbuscular Mycorrhizal Symbiosis in Salt-Tolerance Species and Halophytes Growing in Salt-Affected Soils of South America. In: Pagano M., Lugo, M. (eds), Mycorrhizal Fungi in South America. Fungal Biology. Springer, Cham, doi:10.1007/978-3-030-15228-4_15.

Bencherif, K., Boutekrabt, A., Fontaine, J., Laruelle, F., Dalpè, Y. and Lounès-Hadj Sahraoui, A. 2015. Impact of soil salinity on arbuscular mycorrhizal fungi biodiversity and microflora biomass associated with Tamarix articulate Vahll rhizosphere in arid and semi-arid Algerian areas. Science of the Total Environment 533:488-494, doi:10.1016/j.scitotenv.2015.07.007.

Berruti, A., Lumini, E., Balestrini, R. and Bianciotto, V. 2016. Arbuscular mycorrhizal fungi as natural biofertilizers: let’s benefit from past successes. Frontiers in Microbiology 6:1559, doi:10.3389/fmicb.2015.01559.

Blaskowski, J. 2020. Taxonomy of arbuscular fungi. Websites: (http://www.zor.zut.edu.pl/ Glomeromycota/Blaszkowski%20 Janusz.html) and (http://www.zor.zut.edu.pl/Glomeromycota/Taxonomy.html), access date: August 2020.

Bueno, M. and Cordovilla, M.d.P. 2021. Plant Growth Regulators Application Enhance Tolerance to Salinity and Benefit the Halophyte Plantago coronopus in Saline Agriculture. Plants 10:1872, doi:10.3390/plants10091872.

Calvão, T., Pessoa, M.F. and Lidon, F.C. 2013. Impact of human activities on coastal vegetation. A review. Emirates Journal of Food and Agriculture 25:926-944, doi:10.9755/ejfa.v25i12.16730.

Camprubi, A., Calvet, C., Cabot, P., Pitet, M. and Estaún, V. 2010. Arbuscular mycorrhizal fungi associated with psammophilic vegetation in Mediterranean coastal sand dunes. Spanish Journal of Agricultural Research 8: S96–S102.

Carvalho, L.M., Correia, P.H. and Martins-Loucao, A. 2001. Arbuscular mycorrhizal fungal propagules in a salt marsh. Mycorrhiza 14:165-170, doi:10.1007/s00572-003-0247-4.

Castillo, C.G., Borie, F., Oehl, F. and Sieverding, E. 2016. Arbuscular mycorrhizal fungi biodiversity: prospecting in Southern- Central zone of Chile. A review. Journal of Soil Science and Plant Nutrition 16:400-422, doi: 10.4067/S0718- 95162016005000036.

Estrada, B., Beltrán-Hermoso, M., Palenzuela, J., Iwase, K., Ruiz-Lozano, J.M., Barea, J.M. and Oehl, F. 2013. Diversity of arbuscular mycorrhizal fungi in the rhizosphere of Asteriscus maritimus (L.) Less., a representative plant species in arid and saline Mediterranean ecosystems. Journal of Arid Environments 97:170-175, doi:10.1016/j.jaridenv.2013.05.019.

Gerdemann, J.W. and Nicolson, T.H. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society 46:235-244.

Gonçalves S. and Romano, A. 2016. The medicinal potential of plants from the genus Plantago (Plantaginaceae). Industrial Crops and Products 83:213-226, doi:10.1016/j.indcrop.2015.12.038.

Guillén, A., Mesquita-Joanes, F., Peris, J.B. and Arrillaga, I. 2019. Effects of environmental and temporal factors on Glomeromycotina spores in sand dunes along the Gulf of Valencia (Spain). Fungal Ecology 140:127-139. doi:10.1016/j.funeco.2018.07.001.

Hart, M.M. and Reader, R.J. 2002. Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytologist 153:335-344, doi:10.1046/j.0028-646x.2001.00312.x.

Hesp, P.A.1991. Ecological processes and plant adaptations on coastal dunes. Journal of Arid Environments 21:165-191, doi:10.1016/S0140-1963(18)30681-5.

Hildebrandt, U., Janetta, K., Ouziad, F., Renne, B., Nawrath, K. and Bothe, H. 2001. Arbuscular mycorrhizal colonization of halophytes in Central European salt marshes. Mycorrhiza 10:175-183, doi:10.1007/s005720000074.

INVAM. 2020. International Culture and Collection of Vesicular and Arbuscular Mycorrhizal Fungi. Davis College of Agriculture, Natural Resources and Design. 4100 Agricultural Sciences Building. Websites: (http://invam.caf.wvu.edu) and (https://invam.wvu.edu/methods/spores), access date: August 2020.

Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K., Barea, J. M. 2003. The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and Fertility of Soils 37:1-16, doi:10.1007/s00374- 002-0546-5.

Jenkins, W.R. 1964. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Report 48:692.

Jiang, Y., Wang, W., Xie, Q., Liu, N., Liu, L., Wang, D., Zhang, X. and Yang, C. 2017. Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi. Science 356:1172-1175, doi:10.1126/science.aam9970.

Johnson, N.C., Zak, D.R., Tilman, D. and Pfleger, F.L. 1991. Dynamics of vesicular arbuscular mycorrhizae during old field succession. Oecologia 86:349-358.

Koske, R.E., Gemma, J.N., Corkidi, L., Sigüenza, C., Rincón, E. 2004. Arbuscular Mycorrhizas in Coastal Dunes. In: Martínez, M.L and Psuty N.P. (eds), Ecological Studies. Vol 171. Coastal Dunes, Ecology and Conservation, Springer Verlag, Berlin Heidelberg, pp 173-187, doi:10.1007/978-3-540-74002-5_11.

Landwehr, M., Hilderbrandt, U., Wilde, P., Nawrath, K., Tóth, T., Biró, B. and Bothe, H. 2002. The arbuscular mycorrhizal fungus Glomus geosporum in European saline, sodic and gypsum soils. Mycorrhiza 12:199-211, doi:10.1007/s00572- 002-0172-y.

Liu, R. and Wang, F. 2003. Selection of appropriate host plants used in trap culture of arbuscular mycorrhizal fungi. Mycorrhiza 13:123-127, doi:10.1007/s00572-002-0207-4.

Maherali, H. 2020. Mutualism as a plant functional trait: linking variation in the mycorrhizal symbiosis to climatic tolerance, geographic range, and population dynamics. International Journal of Plant Sciences 181:9-19, doi:10.1086/706187.

Maun, M.A. 2009. The Biology of Coastal Sand Dunes. Oxford Univ. Press, Oxford, U.K.

Melo, C.D., Walker, C., Krüger, C., Borges, P.A.V., Luna, S., Mendonça, D., Fonseca, H.M.A.C. and Machado, A.C. 2019. Environmental factors driving arbuscular mycorrhizal fungal communities associated with endemic woody plant Picconia azorica on native forest of Azores. Annals of Microbiology 69:1309-1327, doi:10.1007/s13213-019- 01535-x.

Milcent, J.P. 2011. Projet de numérisation de la flore de L'Abbé Coste, Tela botanica.

Nehila, A., Ighilhariz, Z., Boukhatem, Z.F. and Bekki, A. 2015. Diversity of Arbuscular Mycorrhizal fungi in two perturbed ecosystems (dune and saline soil) in west Algeria. International Journal of Agriculture and Crop Sciences 8:380-387.

Oliveira, R.S., Vosátka, M., Dodd, J.C. and Castro, P.M.L. 2005. Studies on the diversity of arbuscular mycorrhizal fungi and the efficacy of two native isolates in a highly alkaline anthropogenic sediment. Mycorrhiza 16:23-31, doi:10.1016/j.chemosphere.2005.01.038.

Panwar, J. and Tarafdar, J.C. 2006. Arbuscular mycorrhizal fungal dynamics under Mitragyna parvifolia (Roxb.) Korth. in Thar Desert. Applied Soil Ecology 34:200-208, doi:10.1016/j.apsoil.2006.02.001.

Schmidt, D., Dítětová, Z., Horváth, A. and Szűcs, P. 2016 Coastal newcomer on motorways: the invasion of Plantago coronopus in Hungary. Studia Botanica Hungarica 47:319-334, doi:10.17110/ StudBot.2016.47.2.319.

Schüßler, A., Schwarzott, D. and Walker, C. 2001. A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycological Research 105:1413-1421, doi:10.1017/S0953756201005196.

Selosse, M.A., Strullu-Derrien C., Martin F.M., Kamoun, S. and Kenrick P. 2015. Plants, fungi and oomycetes: a 400-million years affair that shapes the biosphere. New Phytologist 206:501-506, doi:10.1111/nph.13371.

Spatafora, J.W., Chang, Y., Benny, G.L., Lazarus, K., Smith, M.E., Berbee, M.L., Bonito, G., Corradi, N., Grigoriev, I., Gryganskyi, A., James, T.Y., O’Donnell, K., Roberson, R.W., Taylor, T.N., Uehling, J., Vilgalys, R. and White M.M. 2016. A phylum level phylogenetic classification of zygomycete fungi based on genome scale data. Mycologia 108:1028-1046, doi:10.3852/16-042.

Stürmer, S.L., Oliveira, L.Z. and Morton, J.B. 2018. Gigasporaceae versus Glomeraceae (phylum Glomeromycota): A biogeographic tale of dominance in maritime sand dunes. Fungal Ecology 32:49-56, doi:10.1016/j.funeco.2017.11.008.

Touati, J., Chliyeh M., Ouazzani Touhami, A., Benkirane R. and Douira, A. 2013. Mycorrhizal status of Lycium europaeum in the coastal dunes of Mehdia (Northwest of Morocco). Journal of Applied Biosciences 71:5728-5741.

Wang, J., Ma S., Wang GG., Xu L., Fu Z., Song J. and Zhang, J. 2021. Arbuscular mycorrhizal fungi communities associated with wild plants in a coastal ecosystem. Journal of Forest Research 32:683-695, doi:10.1007/s11676-020-01127-5.








How to Cite

Tabti, S., & Bendimered-Mouri, F. Z. (2022). Diversity of arbuscular mycorrhizal fungi in the rhizosphere of Plantago coronopus in Northwestern Algerian coast. Journal of Degraded and Mining Lands Management, 9(2), 3397–3404. https://doi.org/10.15243/jdmlm.2022.092.3397



Research Article